Journal: Oncology reports

Article Title: The mobilization, recruitment and contribution of bone marrow-derived endothelial progenitor cells to the tumor neovascularization occur at an early stage and throughout the entire process of hepatocellular carcinoma growth.

doi: 10.3892/or.2012.1944

Figure Lengend Snippet: Figure 4. The expression of ICAM1, VCAM1, VEGF in TT vs TF. (A) Immunohistochemistry shows the expression of ICAM1, VCAM1, VEGF in TT was much more than that in TF. Dotted lines show the tumor position. Brown particles, positive products of ICAM1, VCAM1, VEGF protein, respectively. Bar, 20 µm (paraffin, 2 µm). (B) Western blotting demonstrated that expression of ICAM1, VCAM1, VEGF in TT was higher than that in TF. The upper bands are the protein, respectively; the lower are the internal standard.

Article Snippet: Target protein levels were measured by immunoblotting with the corresponding antibodies: rabbit anti-mouse actin (1:800, Beyotime, China), rabbit anti-mouse ICAM1 (1:1000, Santa Cruz), rabbit anti-mouse VEGF (1:1000) and goat anti-mouse VCAM1 (1:1000, Abcam).

Techniques: Expressing, Immunohistochemistry, Western Blot